师资力量

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博士/副教授,2005年四川大学化工学院获工学学士学位,同年进入中国科学院过程工程研究所硕博连读。2011年中科院获化学工程与技术工学博士学位,师从邢建民研究员和苏志国研究员,加入重庆大学。2017年4月至今,重庆市科学技术委员会高新技术发展及产业化处副处长(挂职)。

现为化学工程与技术方向硕士生导师,美国化学工程师学会会员、美国微生物学会会员、中国功能材料学会会员、美国旅美专家协会会员。2013年10月-2015年10月在有“南方哈佛”之称的美国莱斯大学(Rice University)化学与生物工程系进行访问学者,博士后及Research Assistant研究(合作导师为Professor Ka-Yiu San),受国家留学基金委和美国NSF、美国农业部项目支持。

联系方式

 dwang@cqu.edu.cn;理科楼LC525,LC522 ;Tel:18523118282;QQ:49330871;微信:maimaitian-minhan

研究方向

围绕国家和企业需求,承担科技攻关和基础研究项目,在生物化工前沿领域开展研究工作:具体为精细化学品合成、合成生物学工程、新能源、新材料

1 生物催化和代谢工程

主要开展从头设计的代谢途径研究,计算机模拟技术和湿实验相结合构建细胞工厂的合成生物学研究;以具有生物活性的酶和全细胞作为催化剂,以可再生生物质为原料的生物催化研究;以生物转化的过程优化为主要研究对象的生物化工工艺研究。

2 天然产物和蛋白分离纯化

利用膜分离、吸附及离子交换、亲和色谱等技术,开展天然产物、基因工程药物、酶等的分离工艺及作用机制研究,实现多种生物活性物质的分离纯化如,白藜芦醇,琥珀酸,蛋白A,重组人粒细胞集落刺激因子,重组人上表皮生长因子等的分离纯化。

3 生物基高分子材料

研究生物基单体二元醇和二元胺等原料性质对聚酯、聚酰胺纤维性能与制备过程稳定性的影响规律,新型生物基聚酯、聚酰胺聚合、成形动力学与结构演化机理;研究新型生物基聚酯、聚酰胺连续聚合与高速纺丝技术,生物基聚酯复合纺丝技术,生物基聚合物阻燃技术,生物基纤维纺织品加工整理技术;生物基聚酯、聚酰胺纤维制备与应用示范。

代表性论文、专著和专利

2017年:

[1] Le Mi, Dandan, Jie Cheng, Dan Wang*, Sa Li, Xuetuan Wei. 2017. Efficient production of free fatty acids from ionic liquid-based acid- or enzyme-catalyzed bamboo hydrolysate. J. Ind. Microbiol. Biotechnol. 44, 419-430. (*通讯作者,IF=2.81)

[2] Wenqin Si, Yuntao Li, Jie Zheng, Shunan Wei*, Dan Wang* 2017. Enhanced hydrolysis of bamboo biomass by chitosan based solid acid catalyst with surfactant addition in ionic liquid. J. Ind. Microbiol. Biotechnol. 44, 419-430. (*通讯作者,IF=3.479)

2016年:

[1] Dandan Qin, Yuanliang Hu, Jie Cheng, Nan Wang, Sha Li, Dan Wang*. Au auto-inducible Escherichia coli strain obtained by adaptively laboratory evolution for fatty acid synthesis from ionic liquid-treated bamboo hydrolystae. Bioresour. Technol., 2016, Sep. 9. doi:10.1016/j.biortech.2016.09.024 (*通讯作者,IF=5.651)

[2] Jie Cheng, Nan Wang, Dezhou Zhao, Dandan Qin, Wenqing Si, Yunfei Tan, Shun’an Wei, Dan Wang*. The enhancement of the hydrolysis of bamboo biomass in ionic liquid with chitosan-based solid acid catalysts immobilized with metal ions. Bioresour. Technol., 2016, Aut. 24. doi:10.1016/j.biortech.2016.08.064 (*通讯作者,IF=5.651, “可再生能源全球创新”网站特别报道)

[3] Wen Jiang, Zhiming Zhou, Dan Wang*, Xiaohua Zhou*, Renyou Tao, Yang Yang, Yexin Shi, Guiluo Zhang, Deyi Wang, Zhen Zhou*. Transglutaminase catalyzed hydrolyzed wheat gliadin grafted with chitosan oligosaccharide and its characterization. Carbohydr. Polym., 2016, Jul. 21. doi:10.1016/j.carbpol.2016.07.097 (*通讯作者,IF=3.479)

2015年:

[1] Dan Wang, Chandresh Thakker, Ping Liu, George N. Benett, Ka-Yiu San*. Efficient production of free fatty acids from soybean meal carbohydrate. Biotechnol. Bioeng., 2015, May 5. doi: 10.1002/bit.25633 (*第一作者,IF=4.16)

[2] Dan Wang, Hui Wu, Chandresh Thakker, Jared Beyersdorf, George N. Benett, Ka-Yiu San*. Efficient free fatty acid production in engineered Escherichia coli strains using soybean oligosaccharides as feedstock. Biotech. Prog., 2015, DOI: 10.1002/btpr. 2092 (*第一作者,IF=1.88)

[3] Jing Wang, Dandan Qin, Baoyun Zhang, Qiang Li, Lichun Dong, Xiaohua Zhou, Dan Wang*.Fine-tuning of ecaA and pepc gene expression increases succinic acid production in Escherichia coli. Appl. Micorbiol. Biotechnol., 2015, DOI: 10.1007/s00253-015-6734-7 (*通讯作者,IF=3.81)

[4] Ka-Yiu San, Hui Wu, Xian Zhang, Dan Wang, KAS-III FREE FA SYNTHESIS, PCT/US2016/018963,国际专利WO2016137897A1

2014年:

[1] Jing Wang, Baoyun Zhang, Jie Zhang, Honghui Wang, Minghui Zhao, Nan Wang, Lichun Dong, Xiaohua Zhou, Dan Wang*. Enhanced succinic acid production and magnesium utilization by overexpression of magnesium transport mgtA in Escherichia coli. Bioresour. Technol., 170: 125-131, 2014 (*通讯作者,IF=5.04)

[2] Nan Wang, Jie Zhang, Honghui Wang, Qiang Li, Sun’an Wei, Dan Wang*. Effects of metal ions on the hydrolysis of bamboo biomass in 1-butyl-3-methylimidazolium chloride with dilute acid as catalyst. Bioresour. Technol., 2014, 173: 399-405 (*通讯作者,IF=5.04)

[3] Jie Zhang, Xiaohua Zhou, Dan Wang*, Xing Zhou, Fang Yun, Shiyu Tan. A comprehensive study of extraction of L-lysine with sec-octylphenoxy acetic acid in sulfonated kerosene. Sep. Sci. Technol. 49, 1349-1356, 2014 (*通讯作者,IF=1.16)

[4] Jie Zhang, Dan Wang*, Jiachuan Pan, Jing Wang, Hongbo Zhao, Qiang Li, Xiaohua Zhou. Efficient resveratrol production by immobilized β-glucosidase on cross-linked chitosan microsphere modified by L-lysine. J. Mol. Catal. B-Enzym. 104, 29-34, 2014 (*通讯作者,IF=2.82)

[5] Wang Honghui, Jiachuan Pan, Dan Wang*, Jing Wang, Nan Wang, Lin Xu, Qiang Li, Xiaohua Zhou. Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain. Biotechnol.Biotec.eq. 2014, accepted (*通讯作者,IF=0.38)

[6] Jing Wang, Qiang Li, Xiaohua Zhou, Dan Wang*. Enhanced succinic acid production and magnesium utilization by overexpression of magnesium transport MgtA in Escherichia coli. 2014, ASM general meeting, Boston, 75-76 (*通讯作者,meeting paper)

[7] Xuetuan Wei, Yinhua Zhou, Jingbang Chen, Dongbo Cai, Dan Wang, Gaofu Qi, Shouwen Chen. Efficient expression of nattokinase in Bacillus licheniformis: host strain construction and signal peptide optimization. J Ind Microbiol Biot, 2014, PMID: 25475755. (IF=2.51)

[8] Jing Wang, Honghui Wang, Nan Wang, Qiang Li, Dan Wang*. Combinatorial engineering of intergenic regions in operons to improve the succinic acid production in Escherichia coli. 2014 Fall Meeting, ASM Branches, 2014/11/6-2014/11/8, pp 58-59, Houston, 2014/11/6. (*通讯作者,meeting paper)

2013年:

[1] 王丹, 王洪辉, 王競, 汪楠, 张杰, 邢建民. 糠醛和5-羟甲基糠醛对大肠杆菌产丁二酸的影响. 中国生物工程学报, 29,1463-1472, 2013 (第一作者)

[2] Jie Zhang, Xiaohua Zhou*, Dan Wang*, Luyan Sang, Yuanhong peng, Shiyu Tan, Qiang Li. High selective separation of flavonoids based on crosslinked chitosan resin grafted by quercetin. Sep. Sci. Technol., 48, 2825-2833, 2013 (*通讯作者,IF=1.16)

[3] Jie Zhang, Xiaohua Zhou*, Dan Wang*, Yingli Wang, Xing Zhou, Honghui Wang, Qiang Li, Shiyu Tan. Studies on the co-immobilized GOD/CAT on cross-linked chitosan microsphere modified by lysine. J. of Mol. Catal. B: Enzym., 97, 80-86, 2013 (*通讯作者, IF=2.82)

[4] Jie Zhang, Xiaohua Zhou*, Zhen Zhou, Yu Su, Ruiya Zuo, Dan Wang*, Shiyu Tan. Preparation and characterization of a novel Ala-Gln dipeptide-Zn2+ complex. Asian J. Chem. 25, 4461-4466, 2013(*通讯作者,IF=0.27)

[5] Jie Zhang, Xiaohua Zhou, Dan Wang*, Yonghong Wang, Lin Chen, Shiyu Tan. Selective separation of L-aromatic amino acid by L-phenylalanine-crosslinked chitosan resin. Asian J. Chem., 25, 9050-9054, 2013 (*通讯作者,IF=0.27)

[6] Dexi Wu, Qiang Li, Dan Wang, Yugang Dong. Enzymatic hydrolysis and succinic acid fermentation from steam-exploded corn stalk at high solid concentration by recombinant Escherichia coli. Appl. Biochem. Biotechnol., 170, 1942-1949, 2013 (第三作者,IF=1.89)

[7] Qiang Li, Jiandu Lei, Rongyue Zhang, Juan Li, Jianmin Xing, Fei Gao, Fangling Gong, Xaofeng Yan, Dan Wang, Zhiguo Su, Guanghui Ma. Efficient decolorization and deproteinization using uniform polymer microspheres in the succinic acid biorefinery from bio-waste cotton (Gossypium hirsutum L.) stalks. Bioresour. Technol., 135, 604-609, 2013 (IF=4.75)

[8]王丹, 王競, 王洪辉, 汪楠, 周小华. 生产丁二酸的重组质粒、基因工程菌的构建及用途. 2013,中国,专利申请号: 201310312492.3 (第一发明人)

[9]王丹, 王洪辉, 汪楠, 王競, 周小华. 木糖醇结晶母液制备葡萄糖酸的反应分离耦合装置和工艺. 2013,中国,专利申请号: 201310312420.9 (第一发明人)

[10]王丹, 汪楠, 魏顺安, 王洪辉, 王競, 张杰, 周小华. 一种降解木质纤维素生成还原糖的方法. 2013,中国,专利申请号: 201310374460.6 (第一发明人)

[11]王丹, 周茂祥, 李茹, 蔡锦华, 颜李秀, 周小华. 一种制备多孔交联壳聚糖微球的新方法. 2013,中国,专利申请号: 201310223533.1 (第一发明人)

2012年:

[1] Jie Zhang, Xiaohua Zhou, H Xu, Luyan Sang, Dan Wang*. Selective Adsorption of Flavonoids on Chitosan Resin Grafted by Quercetin. Proc. - Int. Conf. Biomed. Eng. Biotechnol., 1668-1671, 2012, Macau, DOI: 10.1109/iCBEB.2012.349 (*通讯作者)

[2] Ziyu Song, Qiang Li, Dan Wang, Jie Zhang, and Jianmin Xing. A novel up-flow inner-cycle anoxic bioreactor (UIAB) system for the treatment of sulfide wastewater from purification of biogas. Water Sci. Technol., 65, 1033-1040, 2012 (第三作者,IF=1.10)

2011年:

[1] Dan Wang, Qiang Li, Maohua Yang, Yunjian Zhang, Zhiguo Su, Jianmin Xing. Efficient production of succinic acid from corn stalk hydrolysates by a recombinant Escherichia coli with ptsG mutation. Process Biochem., 46, 365-371, 2011 (第一作者,IF=3.34)

[2] Dan Wang, Qiang Li, Ziyu Song, Wei Zhou, Zhiguo Su, Jianmin Xing. High cell density fermentation via a metabolically engineered Escherichia coli for the enhanced production of succinic acid. J. Chem. Technol. Biotechnol., 86, 512-518, 2011 (第一作者,IF=2.05)

[3] Qiang Li*, Dan Wang*, Guangyu Hu, Jianmin Xing, Zhiguo Su. Integrated bioprocess for high-efficiency production of succinic acid in an expanded-bed adsoption system. Biochem. Eng. J., 56, 150-157, 2011 (共同一作,IF=2.60)

[4] Zelong Wang, Dan Wang, Qiang Li, Wangliang Li, Huang Tang and Jianmin Xing. Enhanced biodesulfurization by expression of dibenzothiophene uptake genes in Rhodococcus erythropolis. World J. Microb. Biot., 27, 1965-1970, 2011(第二作者,IF=1.53)

[5]王丹, 黄小栩, 卢浩, 王孟, 周小华. 一种处理木糖母液的方法. 2011,中国,专利申请号: 201110420250.7 (第一发明人)

[6] 李强, 邢建民, 王丹, 宋子煜, 张云剑. 一种利用离子交换树脂对丁二酸发酵液进行脱色的方法. 2011,中国,专利申请号:201110029752.7 (第三发明人)

[7] 李强, 邢建民, 王丹, 宋子煜, 张云剑. 一种连续结晶生物制造丁二酸的装置及方法. 2011,中国,专利申请号:201110035318.X (第三发明人)

[8] 邢建民,周威,李强,李望良,宋子煜,王丹. 一种具有硫酸盐还原功能的柠檬酸杆菌及其应用. 2011,中国,专利申请号:201110100429.4

2010年:

[1] Dan Wang, Qiang Li, Yu Mao, Jianmin Xing, Zhiguo Su. High-level succinic acid production and yield by lactose-induced expression of phosphoenolpyruvate carboxylase in ptsG mutant Escherichia coli. Appl.Microbiol. Biotechnol., 87, 2025-2035, 2010 (第一作者,IF=3.26)

[2] Qiang Li, Dan Wang, Ziyu Song, Wei Zhou, Yong Wu, Jianmin Xing, Zhiguo Su. Dual-phase fermentation enables Actinobacillus succinogenes 130ZT to be a potential role for high-level lactate production from the bioresource. Bioresource Technol., 101, 7665-7667, 2010 (第二作者,IF=4.75)

[3] Qiang Li, Dan Wang, Yunjian Li, Yunjian Zhang, Jianmin Xing, Zhiguo Su. One step recovery of succinic acid from fermentation broths by crystallization. Sep. Purif. Technol., 72, 294-300, 2010 (第二作者,IF=2.89)

[4] Qiang Li, Dan Wang, Jianmin Xing, Zhiguo Su. Kinetic evaluation of products inhibition to succinic acid producers Escherichia coli NZN111, AFP111 BL21 and Actinobacillus succinogenes 130ZT. J. Microbiol., 48, 290-296, 2010 (第二作者,IF=1.26)

[5] Qiang Li, Maohua Yang, Dan Wang, Wangliang Li, Yong Wu, Yunjian Zhang, Jianmin Xing, Zhiguo Su. Efficient conversion of crop stalk wastes into succinic acid production by Actinobacillus succinogenes. Bioresour. Technol., 101, 3292-3294, 2010 (第三作者,IF=4.75)

[6] Qiang Li, Wangliang Li, Dan Wang, Binbin Liu, Huang Tang, Maohua Yang, Jianmin Xing, Zhiguo Su. pH Neutralization while Succinic Acid Adsorption onto Anion-Exchange Resins. Appl. Biochem. Biotechnol., 160: 428-445, 2010 (第三作者,IF=1.89)

[7] 毛雨,王丹, 黄占斌, 邢建民. 微生物原生质体融合育种技术及其应用.中国生物工程杂志, 30, 93-97, 2010

[8] 毛雨,王丹,李强,邢建民,黄占斌. 产琥珀酸放线杆菌的原生质体制备与再生.中国生物工程杂志, 30, 103-108, 2010

[9] D. Wang, Q. Li, Z. Su, J. Xing. Effects of coexpression of carbonic anhydrase and phosphoenolpyruvate carboxylase on succinic acid production in Escherichia coli. The 3rd International Symposium on Advance Biological Engineering and Scienc. ISABES-3, 2010, 6. 26-6. 28, Beijing, China. (第一作者,meeting paper)

[10] D. Wang, Z. Su, J. Xing. Efficient production of succinic acid from corn stalk hydrolysates by a recombinant Escherichia coli with ptsG mutation. The 2010 International Conference on Biomass Energy and Technologies. ICBT2010, 2010, 8.21-8.23, Beijing, China. (第一作者,meeting paper)

[11] D. Wang, Q. Li, Z. Song, W. Zhou, J. Xing, Z. Su. High cell density fermentation of a metabolically engineered Escherichia coli in fed-batch cultivation for the production of succinic acid. The 2010 International Conference on Biomass Energy and Technologies. ICBT2010, 2010, 8.21-8.23, Beijing, China. (第一作者,meeting paper)

2009及之前:

[1] Dan Wang, Qiang Li, Wangliang Li, Jianmin Xing, Zhiguo Su. Improvement of succinate production by overexpression of a cyanobacterial carbonic anhydrase in Escherichia coli. Enzyme Microbial. Technol., 45, 491-497, 2009 (第一作者,IF=2.87)

[2] Wangliang Li, Huang Tang, Qingfen Liu, Jianmin Xing, Qiang Li, Dan Wang, Maohua Yang, Xin Li, Huizhou Liu. Deep desulfurization of diesel by integrating adsorption and microbial method. Biochem. Eng. J., 44, 297-301, 2009 (第三作者,IF=2.60)

[3] Dan Wang, Qiang Li, Wangliang Li, Jianmin Xing, Zhiguo Su. Overexpression of a cyanobacterial carbonic anhydrase in Escherichia coli enhances succinic acid production. J. Biotechnol. 136S, S26-S27, 2008 (第一作者,IF=3.15)

[4] 王丹, 毛雨, 马兰, 李强, 李望良, 邢建民, 苏志国. 乳糖诱导丙酮酸羧化酶基因在大肠杆菌中的表达及对琥珀酸产量的影响. 生物工程学报, 25, 1338-1344, 2009

[5] 马婕, 王丹, 李强, 邢建民, 刘会洲. 基因工程大肠杆菌合成γ-聚谷氨酸. 过程工程学报, 9, 791-795, 2009

[6] 张玉秀,王姣,王丹,齐峰. 大肠杆菌产琥珀酸基因工程研究进展. 中国生物工程杂志, 29, 102-107, 2009

[7] D. Wang, Q. Li, W. Li, M. Yang, J. Xing, Z. Su. Effects of carbonic anhydrase on succinic acid production in Escherichia coli. The 2nd International Symposium on Advance Biological Engineering and Scienc. ISABES-2, 2008, 3. 31-4. 1, Beijing, China. (第一作者,meeting paper)

[8] D. Wang, Q. Li, W. Li, Q. Liu, J. Xing, Z. Su. Overexpression of a cyanobacterial carbonic anhydrase in Escherichia coli enhances succinic acid production. The 13th International Biotechnology Symposium & Exhibition. IBS-2008, 2008, 10.12-10.17, Dalian, China. (第一作者,meeting paper)

[9] D. Wang, J. Wang, Q. Li, W. Li, J. Xing, Z. Su. Enhanced succinate production by lactose-induced expression of phosphoenolpyruvate carboxylase in △ptsG mutant E. coli. The 5th sino-US Joint Conference of Chemical Engineering, 2009, 10.13-10.16, Beijing, China. (第一作者,meeting paper)

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